Gentian Violet USP

Gentian Violet is a blue, aniline-derived dye. Gentian Violet (also known as methyl violet 10B or hexamethyl pararosaniline chloride) is a triarylmethane dye used as a histological stain and in Gram’s method of classifying bacteria.


Gentian violet is not used as a textile dye. Instead, it is used to dye paper and as a component of navy blue and black inks for printing, ball-point pens, and ink-jet printers. It is also used to colorize diverse products such as antifreezes, detergents, and leather. Gentian violet has antibacterial, antifungal properties.


Chemical Name: Gentian Violet
Molecular Formula: C25H30CIN3
Molecular Weight: 407.98 g/mol
CAS No:  548-62-9


General Specifications

Test Specification
Characters It occurs as a dark green powder or greenish, glistening pieces having a metallic luster, and having not more than a faint odor

Test (A)

Test (B)

Test (C)


(a) Specified Test.

(b)Specified Test. A deep blue precipitate is formed

(c)Specified Test. A blue color is produced at the zone of contact.

Solubility Soluble in alcohol, in glycerin, and in chloroform; sparingly soluble in water; insoluble in ether
Water determination Not more than 7.5%
Residue on ignition Not more than 1.5%
Alcohol insoluble substances Not more than 1.0%
Arsenic Not more than 10ppm
Lead Not more than 30ppm
Zinc Not more than 0.05%
Organic impurities Not more than 1.0%
Residual solvents Meets the requirements
Assay Not less than 96.0% and not more than 100.5% on the anhydrous basis.


Shelf life, Storage and Packing

It should be stored in a segregated area in a cool and ventilated place away from combustible materials. Keep in a tightly closed container, sealed until ready for use. It is packed in 25kg or 50kg HDPE bags or drums as required.


GLP, cGMP (FDA) Approved, ISO 9001, ISO 14001, ISO 45001, ISO 17025, ISO 22000, FSSC 22000, FSSAI, Halal and Kosher certified.
We observe WHO Good manufacturing practice and Good laboratory practice.

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